TABLE 3.
Oligonucleotide primers used in the reverse transcription-qPCR analysis and respective annealing temperature (°C), PCR efficiency (%), accession number and reference.
| Gene | Primer sequence 5′-3′ | Annealing T (°C) | PCR efficiency (%) | Accession number | References | |
| βactin | F | TGCGTGACATCAAGGAGAAG | 58 | 91 | X89920.1 | Avella et al., 2010 |
| R | CAGGACTCCATACCGAGGAA | |||||
| ef1α | F | CTGTCAAGGAAATCCGTCGT | 60 | 100 | AF184170.1 | Estruch et al., 2018 |
| R | TGACCTGAGCGTTGAAGTTG | |||||
| rps18 | F | AGGGTGTTGGCAGACGTTAC | 60 | 101 | AM490061.1 | Salmerón et al., 2016 |
| R | CTTCTGCCTGTTGAGGAACC | |||||
| Igf2 | F | TGGGATCGTAGAGGAGTGTTGT | 62 | 92 | AY996778.1 | Benedito-Palos et al., 2007 |
| R | CTGTAGAGAGGTGGCCGACA | |||||
| selk | F | TGTACGTGTCCAACGGTCAG | 60 | 95 | XM_030423889.1 | – |
| R | TGAATTCCACTGCTCCCCAG | |||||
| selp | F | CATTTATGACAGGTGTGGCCG | 62 | 94 | XM_030416800.1 | – |
| R | CGCTTGCAGTACGCATCTTT | |||||
| dio1 | F | GACTCGTCAGGGACTTCAGC | 60 | 97 | DQ888894.1 | – |
| R | GAGCCTCTCAGGCATAGCAC | |||||
| dio2 | F | GGCAGTTGGTTGAGGACTTC | 60 | 91 | DQ888895.1 | – |
| R | TCCATACAGTCAGCCACCAG | |||||
| gpx1a | F | TGGAGAAGGTGGATGTGAATG | 58 | 92 | KC201352.1 | Malandrakis et al., 2014 |
| R | GCCATCAGGACCAACAAGG | |||||
| gpx1b | F | GCGTTACAGCAGGAGGTT | 60 | 90 | KC201353.1 | Malandrakis et al., 2014 |
| R | TGAGCACATCTGGTCATTCA | |||||
| gpx4b | F | ACGGCTTGTCTGTAAACTC | 60 | 99 | KC201354.1 | Malandrakis et al., 2014 |
| R | GCTGGTGAATGTCCTCTC | |||||
| cpt1a | F | GTGCCTTCGTTCGTTCCATGATC | 62 | 99 | JQ308822.1 | Betancor et al., 2016 |
| R | TGATGCTTATCTGCTGCCTGTTTG | |||||
| Fas | F | TGCCATTGCCATAGCACTCA | 58 | 92 | JQ277708.1 | Houston et al., 2017 |
| R | ACCTTTGCCCTTTGTGTGGA | |||||
| srebp1 | F | AGGGCTGACCACAACGTCTCCTCTCC | 60 | 93 | JQ277709.1 | Houston et al., 2017 |
| R | GCTGTACGTGGGATGTGATGGTTTGGG | |||||
| fads2 | F | GCAGGCGGAGAGCGACGGTCTGTTCC | 60 | 99 | AY055749.1 | Betancor et al., 2016 |
| R | AGCAGGATGTGACCCAGGTGGAGGCAGAAG | |||||
| elovl5 | F | CCTCCTGGTGCTCTACAAT | 60 | 92 | AY660879.1 | Betancor et al., 2016 |
| R | GTGAGTGTCCTGGCAGTA | |||||
Reference genes: βactin, actin beta; ef1α, elongation factor 1 alpha and rps18, ribosomal protein S18. Target genes related to growth: igf2, insulin-like growth factor 2; selenoprotein genes: selk, selenoprotein K and selp, selenoprotein P; genes involved in iodine metabolism: dio1, iodothyronine deiodinase 1 and dio2, iodothyronine deiodinase 2; genes involved in oxidative stress: gpx1a, glutathione peroxidase 1a; gpx1b, glutathione peroxidase 1b and gpx4b, glutathione peroxidase 4b; and genes associated with lipid metabolism: cpt1a, carnitine palmitoyl transferase 1a; fas, fatty acid synthase; srebp1, sterol regulatory element binding protein 1; fads2, fatty acid desaturase 2; and elovl5, elongation of very long chain fatty acids protein 5.