TABLE 6.
Whole-body fatty acid composition (% total fatty acids) and retention (% feed intake).
| CTRL | AD1 | AD2 | AD3 | P | |
| Fatty acids | |||||
| 14:0 | 3.08 ± 0.33ab | 2.71 ± 0.31b | 3.27 ± 0.17ab | 3.59 ± 0.20a | 0.02 |
| 16:0 | 22.57 ± 2.45 | 20.57 ± 2.57 | 23.38 ± 1.29 | 26.13 ± 2.06 | 0.07 |
| 18:0 | 5.54 ± 0.96 | 4.89 ± 0.65 | 5.50 ± 0.18 | 6.33 ± 0.76 | 0.17 |
| 1Σ SFA | 32.36 ± 3.72 | 29.41 ± 3.58 | 33.46 ± 1.80 | 37.30 ± 2.91 | 0.07 |
| 16:1 n-7 | 4.04 ± 0.72 | 4.45 ± 0.45 | 4.36 ± 0.25 | 4.26 ± 0.61 | 0.81 |
| 18:1 n-9 c (OA) | 35.42 ± 0.68 | 35.16 ± 0.81 | 34.17 ± 1.51 | 34.22 ± 0.51 | 0.33 |
| 20:1 n-9 | 1.60 ± 0.06 | 1.34 ± 0.16 | 1.53 ± 0.03 | 1.55 ± 0.06 | 0.11 |
| 2Σ MUFA | 41.95 ± 1.04 | 41.91 ± 1.10 | 40.91 ± 1.63 | 40.84 ± 0.97 | 0.55 |
| 18:2 n-6 c (LA) | 16.06 ± 2.05 | 16.79 ± 1.49 | 15.38 ± 0.30 | 13.43 ± 1.62 | 0.11 |
| 18:3 n-3 (ALA) | 3.45 ± 0.52ab | 3.83 ± 0.43a | 3.24 ± 0.15ab | 2.68 ± 0.45b | 0.04 |
| 20:5 n-3 (EPA) | 1.74 ± 0.13ab | 1.78 ± 0.17a | 1.74 ± 0.07ab | 1.36 ± 0.20b | 0.03 |
| 22:6 n-3 (DHA) | 2.92 ± 0.28c | 4.36 ± 0.38a | 3.70 ± 0.14b | 2.85 ± 0.04c | <0.001 |
| aEPA + DHA | 4.66 ± 0.33bc | 6.14 ± 0.54a | 5.44 ± 0.15ab | 4.21 ± 0.16c | <0.001 |
| 3Σ PUFA | 25.40 ± 2.66ab | 28.08 ± 2.52a | 25.22 ± 0.32ab | 21.46 ± 2.25b | 0.03 |
| Retention (% of Intake) | |||||
| 14:0 | 83.50 ± 14.23 | 48.73 ± 12.11 | 57.42 ± 14.73 | 62.89 ± 19.40 | 0.11 |
| 16:0 | 117.57 ± 27.58a | 52.11 ± 16.09b | 64.48 ± 17.31ab | 74.37 ± 27.02ab | 0.04 |
| 18:0 | 115.21 ± 35.27 | 68.88 ± 25.12 | 86.55 ± 33.97 | 105.96 ± 47.77 | 0.46 |
| 1Σ SFA | 108.46 ± 24.88 | 52.84 ± 15.81 | 64.73 ± 18.17 | 74.22 ± 27.11 | 0.07 |
| 16:1 n-7 | 49.22 ± 39.50 | 40.99 ± 17.64 | 41.09 ± 38.66 | 32.04 ± 30.17 | 0.93 |
| 18:1 n-9 c (OA) | 64.32 ± 25.10 | 47.18 ± 8.08 | 53.98 ± 31.26 | 49.46 ± 26.79 | 0.83 |
| 20:1 n-9 | 63.24 ± 16.75 | 29.95 ± 17.95 | 51.24 ± 24.26 | 50.51 ± 28.64 | 0.39 |
| 2Σ MUFA | 57.52 ± 23.11 | 41.95 ± 7.44 | 47.06 ± 28.04 | 42.71 ± 24.11 | 0.81 |
| 18:2 n-6 c (LA) | 44.95 ± 21.31 | 37.05 ± 10.12 | 35.39 ± 15.89 | 22.06 ± 11.75 | 0.39 |
| 18:3 n-3 (ALA) | 42.64 ± 17.35 | 41.27 ± 9.09 | 35.91 ± 14.12 | 24.26 ± 7.38 | 0.34 |
| 20:5 n-3 (EPA) | 30.47 ± 10.57 | 30.34 ± 5.87 | 25.22 ± 9.47 | 15.59 ± 4.38 | 0.16 |
| 22:6 n-3 (DHA) | 47.62 ± 27.03 | 48.78 ± 11.15 | 33.94 ± 14.39 | 16.75 ± 8.83 | 0.15 |
| 3Σ PUFA | 42.42 ± 18.81 | 37.89 ± 9.27 | 32.66 ± 13.69 | 20.29 ± 9.24 | 0.28 |
Values presented as mean ± SD, with three pooled samples per treatment of six fish per tank for final whole-body composition and two pooled samples of six fish per tank for initial whole-body composition. Different superscript letters in a row denote significant differences among treatments.
1Σ SFA is the sum of saturated fatty acids and also includes 15:0, 17:0, 20:0, 22:0 and 24:0;
2Σ MUFA is the sum of monounsaturated fatty acids and also includes 17:1 n-7, 22:1 n-9 and 24:1 n-9;
3Σ PUFA is the sum of polyunsaturated fatty acids and also includes 18:3 n-6, 20:2 n-6, 20:3 n-6 and 20:4 n-6;
aEPA + DHA = eicosapentaenoic acid + docosahexaenoic acid. OA: oleic acid; LA: linoleic acid; ALA: α-linolenic acid.