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. 2022 Jan 13;9:785979. doi: 10.3389/fcell.2021.785979

FIGURE 2.

FIGURE 2

Dual inhibition of mTORC1 and mTORC2 reduces surviving fraction but not colony-forming ability. Treatment with OSI-027 (250 and 500 nM) for 48 h decreased the surviving fraction significantly in CCA cells HuCCA1 over 2 weeks (p < 0.03) (A). Representative images (B–D) show the characteristic morphology of the para-, mero- and holoclones of HuCCT1. The morphology of the colonies and the colony-forming ability of HuCCA1 (E) and HuCCT1 (F) did not alter significantly in the distribution of para-, mero- and holoclones. Asterisks (* p ≤ 0.05) indicate significance between treatment and control. Experiments were performed in triplicates. Scale bar indicates 500 µm.