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. 2019 Mar 13;33(6):7555–7562. doi: 10.1096/fj.201900014R

Figure 6.

Figure 6

A2AR stimulation does not increase p‐S552 β‐catenin levels after Akt knockdown. A) Liposomal transfection of siRNA in MC3T3C‐E1 cells (4 h). Scale bar, 200 µm. B) Superimposition of bright‐field and fluorescent images. Scale bar, 200 µm. C) Transfection of siRNA for Akt‐1 reduced cellular p‐S473 Akt levels. *P < 0.05, Student's t test. D) Stimulation of A2AR with CGS21680 (1 µM) for 15 min increased cellular p‐S552 β‐catenin levels in the cells transfected with scrambled siRNA but had no effect on p‐S552 β‐catenin levels in the Akt‐1 siRNA‐transfected cells. *P < 0.05, Student's t test.