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. Author manuscript; available in PMC: 2022 Jan 27.
Published in final edited form as: Cell Rep. 2022 Jan 18;38(3):110255. doi: 10.1016/j.celrep.2021.110255

Figure 4. Synaptotagmin-1 modulates baclofen-induced inhibition of spontaneous glutamate release.

Figure 4.

(A) Representative immunoblot of protein samples obtained from control group (infected by lentiviral particles carrying empty backbone) and Syt1 KD group (infected by lentiviral particles carrying Syt1 shRNA construct) and its quantification (two-tailed, non-paired t test; WT n = 6; Syt1 KD n = 6).

(B) Representative evoked inhibitory postsynaptic currents (eIPSCs) elicited by single stimulus at baseline and during 10 μM BAC perfusion. Note that the eIPSCs became smaller in amplitude and grossly asynchronous when Syt1 was knocked down (Syt1 KD). The effect of BAC on eIPSC amplitudes was quantified over time and normalized to baseline. At steady state, the mean values were horizontally fitted and fits were compared (WT n = 14; Syt1 KD n = 14).

(C) Representative traces of mEPSCs recorded at baseline and during 10 μM BAC perfusion along with quantitative analysis of event frequencies and amplitudes at baseline for WT control and Syt1 KD (two-tailed, non-paired t test; WT n = 14; Syt1 KD n = 14).

(D) Quantitative analyses of mEPSC frequencies at baseline and during 10 μM BAC perfusion for WT control and Syt1 KD (two-tailed, paired t test; WT n = 14; Syt1 KD n = 14), along with % decrease in mEPSC frequency upon BAC perfusion (two-tailed, non-paired t test; WT n = 14; Syt1 KD n = 14).

(E) Representative traces of mIPSCs recorded at baseline and during 10 μM BAC perfusion along with quantitative analysis of event frequencies and amplitudes at baseline for WT control and Syt1 KD (two-tailed, non-paired t test; WT n = 12; Syt1 KD n = 12).

(F) Quantitative analyses of mIPSC frequencies at baseline and during 10 μM BAC perfusion for WT control and Syt1 KD (two-tailed, paired t test; WT n = 12; Syt1 KD n = 12), along with % decrease in mIPSC frequency upon BAC perfusion (two-tailed, non-paired t test; WT n = 12; Syt1 KD n = 12).

All panels: significance levels are stated as follows: *p < 0.05; **p < 0.01; ***p < 0.001; and ****p < 0.0001.