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. 2022 Jan 13;11:828861. doi: 10.3389/fonc.2021.828861

Figure 4.

Figure 4

YY1 stimulated HCC cell culture media accelerated endothelial cells neovascularization. (A) HUVECs and HAECs (red) and HepG2 cells (green) co-cultured in a 1:2 ratio and formed three-dimensional spheroids. Images were taken with a laser scanning confocal microscope, scale bar = 50 μm. (B) Representative image (left) of the formation of HUVECs and HAECs tubes following an incubation with supernatants collected from the indicated cells. Tube formation quantification were analyzed (right). Scale bar = 50 μm. (C) HUVECs and HAECs migration were detected after an incubation with supernatants collected from the indicated cells. (D) HUVECs and HAECs invasion were detected following an incubation with supernatants collected from the indicated cells. Scale bar = 20 μm. (E) WB analyzed pVEGFR2 expression in HUVECs and HAECs treated with conditioned media. (F) Immunofluorescence of pVEGFR2 expression in HUVECs and HAECs treated with conditioned media. Scale bar = 10 μm. *P < 0.05, **P < 0.01.