Table 1.
Transcriptomic changes consistent with reduced Notch signaling and increased goblet cell differentiation in WT enteroids treated with 100 µM Pam3Csk4 for 72 h
| Gene | Means | ± SE | P Value |
|---|---|---|---|
| Increased mRNA expression | |||
| Relative to untreated enteroids | |||
| Muc1 | 3.61 | ± 0.78 | 0.0055 |
| Muc2 | 1.32 | ± 0.33 | 0.0097 |
| Spdef | 0.67 | ± 0.18 | 0.0064 |
| Agr2 | 0.65 | ± 0.13 | 0.0046 |
| c-Fos | 0.55 | ± 0.09 | 0.0055 |
| Creb3l1 | 0.51 | ± 0.20 | 0.0356 |
| Atoh1 | 0.33 | ± 0.14 | 0.0099 |
| Gfi1 | 0.26 | ± 0.18 | 0.0055 |
| Decreased mRNA expression | |||
| Relative to untreated enteroids | |||
| Notch1 | −1.10 | ± 0.36 | 0.0340 |
| Sox9 | −0.88 | ± 0.30 | 0.0340 |
| Neurog3 | −0.57 | ± 0.12 | 0.0340 |
| Foxa2 | −0.48 | ± 0.16 | 0.0340 |
Effect of Pam3Csk4 treatment on WT enteroids on mRNA expression of epithelial differentiation markers. Enteroids were treated for 72 h with 100 µg/mL of Pam3Csk4 before collection for mRNA measurement by qRT-PCR; n = 6 WT mice (4 males, 2 females). Genes with unchanged expression are shown in Supplemental Table S2. WT, wild-type.