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Isolation of the fbn2 knockout mutant. (A) Schematic illustration of the genomic DNA fragment containing the FBN2 gene. The T-DNA insertion in the first exon of FBN2 found in the SALK_124590 line is shown. The primers used in the analysis of wild-type (WT) or mutant FBN2 alleles are indicated. (B) PCR analysis of a homozygous fbn2 mutant plant. Only the mutant allele is detected. (C) Immunoblot analysis of leaf extracts of WT and fbn2 mutant plants using anti-FBN2 antibodies.
