Fig. 3.

Anthocyanin accumulation in fbn mutants. (A) Knockout mutants fbn2, fbn1a-1b, and fbn1a-1b-2 and WT plants were sown in soil and cultivated under normal conditions for 3 weeks. After this period, the plants were subjected to stress conditions (600 µmol m⁻² s⁻¹ light intensity and 10 °C temperature). Twelve rosette leaves from four plants per line were harvested after 0, 1, and 3 weeks of treatment, and the concentrations of anthocyanins were determined. (B) Plants cultivated under normal conditions were subjected to stress as described in (A) for 1 week with or without the addition of 2mM JA every 3 d, and the concentrations of anthocyanins were determined. In both panels, the values are presented as means ±SD. Two-way ANOVA was performed using PRISM software version 6.0. Tukey’s HSD test was used as a post hoc test. Significant differences (P<0.01) between mean values are indicated with different letters.