Fig. 4.

Glucosyltransferase activity of purified CESAs (A) CtCESA1 isolated from heterologous expression in insect cell and yeast cells. (B) CtCESA1 activity in GDN detergent from (A) compared with PttCESA8 and BCSA/BCSB reconstituted proteins or E. coli total lipids, respectively, normalized by protein concentration. UDP-glucose ³H-labeled tracer activity assays was measured with either nothing (Ø), Ca²⁺ (ca), Mg²⁺ (mg), or Mn²⁺ (mn). DPM=disintegrations per minute. Significantly different values are indicated with letters. All measurements were performed in triplicate and represented as one average above each boxplot. Statistical differences determined with Welch’s ANOVA followed by Tukey post-hoc analysis (P<0.01).