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. Author manuscript; available in PMC: 2022 Dec 1.
Published in final edited form as: Breast Cancer Res Treat. 2021 Sep 22;190(3):373–387. doi: 10.1007/s10549-021-06398-y

Fig. 2. Methionine restriction induces TXNRD1 by a ROS-, NRF2- and ATF4-dependent mechanism.

Fig. 2

A, Immunoblot of p-NRF2, ATF4 and TXNRD1 expression in GILM2 (left) and MDA-MB-468 (right) cells cultured in control or MR media for 24 hours in the presence or absence of NAC (2 mM). B, Immunoblot of p-NRF2, ATF4 and TXNRD1 expression in WT, ATF4−/− and NRF2−/− MEFs grown in MR media for the indicated number of hours. C, TXNRD activity (expressed as percentage of time t=0) was determined in the indicated MEFs cultured in control or MR media for 48 hours (mean ± SEM, n = 3). ***, P < 0.001 vs. control