TABLE 2.
M38-P MICs for 181 opportunistic molds determined by the E-test and the NCCLS M38-P methoda
| Fungus (no. of isolates tested) | Antifungal Agentb | Incubation time (h) | MIC (μg/ml) determined by:
|
|||||
|---|---|---|---|---|---|---|---|---|
| E-test
|
NCCLS M38-P method
|
|||||||
| Range | Geometric mean | 90% | Range | Geometric Mean | 90% | |||
| A. flavus (16) | A | 24 | 0.25–>8 | 3.3 | >8 | |||
| 48 | 0.5–>8 | >8 | >8 | 0.5–4 | 1.26 | 2 | ||
| I | 24 | 0.06–0.25 | 0.14 | 0.25 | ||||
| 48 | 0.12–1.0 | 0.25 | 0.5 | 0.03–0.25 | 0.11 | 0.25 | ||
| A. fumigatus (69) | A | 24 | 0.25–8 | 1.39 | 2 | |||
| 48 | 0.25–>8 | 5.2 | >8 | 0.25–4 | 1.2 | 1.0 | ||
| I | 24 | 0.06–>8 | 0.84 | 2 | ||||
| 48 | 0.12–>8 | 1.57 | 4 | 0.03–>8 | 0.71 | 0.5 | ||
| A. nidulans (12) | A | 24 | 0.25–>8 | 1.5 | 2 | |||
| 48 | 0.5–>8 | 6.1 | >8 | 0.5–4 | 0.88 | 2 | ||
| I | 24 | 0.06–0.25 | 0.12 | 0.25 | ||||
| 48 | 0.12–1.0 | 0.15 | 0.5 | 0.06–0.25 | 0.16 | 0.25 | ||
| A. niger (10) | A | 24 | 0.5–1.0 | 0.75 | 1.0 | |||
| 48 | 1.0 | 1.0 | 1.0 | 0.5–1.0 | 0.7 | 1.0 | ||
| I | 24 | 0.5 | 0.5 | 0.5 | ||||
| 48 | 1.0 | 1.0 | 1.0 | 0.12–1.0 | 0.48 | 1.0 | ||
| A. terreus (16) | A | 24 | 0.25–4 | 2.13 | 4 | |||
| 48 | 0.25–8 | 2.85 | 4 | 0.5–4 | 1.4 | 4 | ||
| I | 24 | 0.01–0.5 | 0.18 | 0.5 | ||||
| 48 | 0.03–0.5 | 0.19 | 0.5 | 0.03–0.5 | 0.14 | 0.25 | ||
| F. oxysporum (6) | A | 24 | 1.0–>8 | >8 | >8 | |||
| 48 | 1.0–>8 | >8 | >8 | 1.0 | 1.0 | 1.0 | ||
| I | 24 | 4–>8 | >8 | >8 | ||||
| 48 | >8 | 1.0–>8 | >8 | >8 | ||||
| F. solani (10) | A | 24 | 0.12–>8 | 2.08 | 8 | |||
| 48 | 0.5–>8 | >8 | >8 | 0.5–4 | 1.62 | 4 | ||
| I | 24 | >8 | ||||||
| 48 | >8 | 8–>8 | >8 | >8 | ||||
| P. lilacinus (4) | A | 48–72 | >8 | >8 | ||||
| I | 48–72 | 2–>8 | 7 | >8 | 2–>8 | 5.7 | >8 | |
| S. apiospermum (10) | A | 48 | 4–8 | 6.7 | 8 | |||
| 72 | 4–8 | 6.7 | 8 | 1–8 | 3.17 | 4 | ||
| I | 48 | 0.25–0.5 | 0.4 | 0.5 | ||||
| 72 | 0.25–0.5 | 0.4 | 0.5 | 0.25–1.0 | 0.85 | 1.0 | ||
| S. prolificans (5) | A | 48 | >8 | 8–>8 | >8 | >8 | ||
| I | 48 | >8 | 4–>8 | >8 | >8 | |||
| T. longibrachiatum (5) | A | 24 | 0.5–1.0 | 0.83 | 1.0 | |||
| 48 | 1.0–4 | 2.2 | 2 | 0.5–2 | 0.87 | 0.5 | ||
| I | 24 | >8 | ||||||
| 48 | >8 | >8 | ||||||
| Dematiaceous molds (18)c | A | 48 | 0.06–4 | 1.0 | 2 | |||
| 72 | 0.12–>8 | 2.74 | 8 | 0.12–4 | 0.85 | 2 | ||
| I | 48 | 0.01–0.12 | 0.06 | 0.12 | ||||
| 72 | 0.01–0.5 | 0.13 | 0.25 | 0.06–2 | 0.37 | 0.5 | ||
a
The NCCLS M38-P broth microdilution method has been described previously (22).
b
A, amphotericin B; I, itraconazole
c
This group included one to three isolates each of Bipolaris spp., C. bantiana, C. cladosporioides, D. constricta var. gallopava, P. parasiticum (P. parasitica), and W. dermatitidis.