TABLE 3.
Distribution of differences in MICs for 181 molds and percent agreement within 3 dilutions for the E-test and the NCCLS M38-P method
| Fungus (no. of isolates tested) | Antifungal agenta | Incubation time (h) or reading no. | No. of isolates for which MICs determined by E-test differed from MICs determined by method by the following dilution:
|
% Agreementb | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| >+2 | >+2 | +1 | 0 | −1 | −2 | >−2 | ||||
| A. flavus (16) | A | 24 | 4 | 3 | 3 | 3 | 1 | 2 | 75 | |
| 48 | 11 | 3 | 1 | 1 | 31.2 | |||||
| I | 24 | 3 | 5 | 5 | 3 | 100 | ||||
| 48 | 7 | 4 | 5 | 100 | ||||||
| A. fumigatus (69) | A | 24 | 4 | 14 | 16 | 22 | 8 | 2 | 97 | |
| 48 | 25 | 15 | 8 | 21 | 63.8 | |||||
| I | 24 | 5 | 11 | 17 | 23 | 7 | 2 | 1 | 90 | |
| 48 | 18 | 12 | 20 | 17 | 2 | 74 | ||||
| A. nidulans (12) | A | 24 | 1 | 2 | 2 | 4 | 1 | 1 | 83.3 | |
| 48 | 5 | 2 | 4 | 1 | 58.3 | |||||
| I | 24 | 1 | 2 | 1 | 5 | 2 | 83.3 | |||
| 48 | 2 | 1 | 1 | 6 | 2 | 83.3 | ||||
| A. niger (10) | A | 24 | 2 | 5 | 3 | 100 | ||||
| 48 | 3 | 7 | 100 | |||||||
| I | 24 | 1 | 9 | 100 | ||||||
| 48 | 1 | 9 | 100 | |||||||
| A. terreus (16) | A | 24 | 2 | 1 | 5 | 3 | 1 | 1 | 77 | |
| 48 | 3 | 4 | 4 | 4 | 1 | 75 | ||||
| I | 24 | 1 | 4 | 3 | 1 | 4 | 92 | |||
| 48 | 2 | 4 | 3 | 2 | 1 | 4 | 88 | |||
| F. oxysporum (6) | A | 24 | 5 | 1 | NAc | |||||
| 48 | 6 | NA | ||||||||
| I | 24 | 2 | 3 | 1 | NA | |||||
| 48 | 6 | NA | ||||||||
| F. solani (10) | A | 24 | 1 | 1 | 2 | 2 | 2 | 2 | 70 | |
| 48 | 6 | 1 | 1 | 2 | 40 | |||||
| I | 24 | 10 | 100 | |||||||
| 48 | 10 | 100 | ||||||||
| P. lilacinus (4) | A | 48–72 | 4 | NA | ||||||
| I | 48–72 | 1 | 2 | 1 | NA | |||||
| S. apiospermum (10) | A | 48 | 3 | 3 | 4 | 100 | ||||
| 72 | 3 | 3 | 4 | 100 | ||||||
| I | 48 | 3 | 4 | 3 | 100 | |||||
| 72 | 3 | 4 | 3 | 100 | ||||||
| S. prolificans (5) | A | 24 | ||||||||
| 48 | 1 | 4 | NA | |||||||
| I | 24 | |||||||||
| 48 | 1 | 4 | NA | |||||||
| T. longibrachiatum (5) | A | 24 | 3 | 2 | NA | |||||
| 48 | 2 | 3 | NA | |||||||
| I | 24 | 5 | NA | |||||||
| 48 | 5 | NA | ||||||||
| Dematiaceous molds (18)d | A | 48 | 1 | 3 | 3 | 5 | 2 | 2 | 93.7 | |
| 72 | 1 | 3 | 4 | 5 | 93.3 | |||||
| I | 48 | 1 | 1 | 3 | 2 | 7 | 7 | 81.2 | ||
| 72 | 1 | 3 | 7 | 2 | 2 | 2 | 83.3 | |||
| All isolates (181) | A | Reading 1 | 14 | 17 | 36 | 42 | 33 | 15 | 6 | 87.7 |
| Reading 2 | 57 | 27 | 22 | 40 | 26 | 8 | 1 | 67.9 | ||
| I | Reading 1 | 7 | 21 | 29 | 63 | 21 | 17 | 5 | 92.7 | |
| Reading 2 | 21 | 26 | 41 | 63 | 15 | 11 | 4 | 86 | ||
a
A, amphotericin B; I, itraconazole.
b
Percent agreement is the percentage of the MIC results obtained by the two methods that were within 3 dilutions.
c
NA, not applicable (too few isolates).
d
Isolates of C. bantiana needed 4 days of incubation.