Figure 5.

Changes in pulmonary fibroblasts with cyclic stretch. A: by flow cytometry, organoids subjected to 20 h of cyclic stretch had approximately double the number of α-smooth muscle actin (αSMA)-positive cells as unstretched and static stretch organoids. n = 4 per group. Kruskal–Wallis P < 0.01 and Dunn’s post hoc test results are shown. There was no overall difference in the total number of PDGFRα-positive cells (B), but the number of αSMA-PDGFRα-double-positive cells (C) was increased in cyclic stretch compared with static or no stretch. D: representative flow cytometry plots of the experiment. PDGFRα, platelet-derived growth factor receptor-α.