Figure 1.

Tys protects against HK-MRSA- or α-toxin induced lung endothelial barrier disruption. Endothelial permeability was monitored using the electric cell-substrate impedance sensing (ECIS) system. HLMVEC (A) or HPAEC (B) on ECIS plates were treated with HK-MRSA (2 × 10⁸/mL) for 6 h or 4 h, respectively. C: HPAECs were treated with α-toxin (150 ng/mL). Tys (1 μM) was added, as indicated by the arrow, and transendothelial resistance values (TERs) were recorded for up to 20 h. Resistance was normalized at the time point before HK-MRSA/α-toxin addition. Depicted are pooled TER tracings and corresponding area under the curve (AUC) quantifications for each condition. n = 3 or 4 independent experiments. One-way ANOVA, ***P < 0.001. α-T, α-toxin; C, control; HK-M, HK-MRSA, heat-killed MRSA; HLMVEC, human lung microvascular endothelial cell; HPAEC, human pulmonary artery endothelial cell; MRSA, methicillin-resistant Staphylococcus aureus.