Figure 2.

Effect of Tys on cytoskeletal remodeling and junctional disruption induced by HK-MRSA. HPAECs were treated with HK-MRSA (2 × 10⁸/mL) for 6 h and then treated with Tys (1 μΜ) for 15 min. Cells were fixed and analyzed by immunofluorescence after staining with VE-cadherin Ab, cortactin Ab, or phalloidin (actin staining). Each staining was performed in a different set of cells. Depicted are representative images from three or more independent experiments. White arrows and triangles indicate peripheral VE-cadherin and cortactin staining, respectively. *Stress fiber formation. Pictures were taken at ×40 magnification. HK-MRSA, heat-killed MRSA; HPAEC, human pulmonary artery endothelial cell; MRSA, methicillin-resistant Staphylococcus aureus.