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. 2021 Dec 8;322(1):L149–L161. doi: 10.1152/ajplung.00100.2021

Figure 3.

Figure 3.

Tys inhibits MLC phosphorylation and RhoA activation induced by HK-MRSA. A and B: HPAECs were treated with HK-MRSA (2 × 108/mL) and Tys (1 μΜ) for 1 h. A: representative Western blots of pMLC and total MLC. B: densitometric analysis of Western blot data. The graph depicts the fold expression of pMLC/MLC. n = 3. C and D: HPAECs were treated with HK-MRSA (2 × 108/mL) and Tys (1 μΜ) for 0.5 h. RhoA activation was evaluated by Rho Activation Assay Biochem Kit. Depicted are representative blots of GTP-RhoA and total RhoA (C) and corresponding densitometric analysis (D). n = 3. E: HPAECs on ECIS plates were pretreated with 10 μM ROCK Inhibitor (Y-27632) for 1 h, then treated with 2 × 108/mL HK-MRSA. TER was monitored over a 10-h period using ECIS. Depicted are pooled TER tracings from three independent experiments. One-way ANOVA (B and D), t test (E). *P < 0.05, **P < 0.01, ***P < 0.001. C, control; ECIS, electric cell-substrate impedance sensing; HK-M, HK-MRSA, heat-killed MRSA; HPAEC, human pulmonary artery endothelial cell; MRSA, methicillin-resistant Staphylococcus aureus; TER, transendothelial electrical resistance; Y, Y-27632.