Figure 4.

Tys inhibits inflammatory signaling induced by HK-MRSA. HPAECs were treated with HK-MRSA (2 × 10⁸/mL) for 1, 2, or 3 h (A); or with or without Tys (1 µM) for 3 h (B). Cell lysates were analyzed by Western blotting. Depicted are representative Western blots of phospho-NF-κB (p-NF-κB) and total NF-κB (pan-NF-κB) and corresponding densitometric analyses. n = 3. C and D: HPAECs were treated with HK-MRSA (2 × 10⁸/mL) with or without Tys (1 µM) for 6 h, and IL-6 (C), or IL-8 (D) levels were detected by ELISA in the cell supernatant. n = 4 independent experiments. One-way ANOVA, *P < 0.05, **P < 0.01, ***P < 0.001. C, control; HK-M, HK-MRSA, heat-killed MRSA; HPAEC, human pulmonary artery endothelial cell; MRSA, methicillin-resistant Staphylococcus aureus.