Figure 5.
Effects of Tys pretreatment on MRSA-induced acute lung injury. Wild-type mice were treated with Tys (0.5 mg/kg, ip) and then challenged with live bacteria-MRSA (1.25 × 108 CFU/mouse, intratracheally, 18 h). Bronchoalveolar lavage (BAL) protein (A), total cell counts (B), and neutrophil percentages were determined (C). D: H&E lung staining. Lung tissues were scanned with a digital slide scanner at ×40 and representative pictures were taken at ×20. Scale bar: 200 μm. Representative images are shown. n = 3–8 mice per condition. One-way ANOVA, *P < 0.05, **P < 0.01, ***P < 0.001. CFU, colony-forming unit; H&E, hematoxylin and eosin; MRSA, methicillin-resistant Staphylococcus aureus.