Figure 3.

Permeability analysis of recombinant protein in vivo. (A) Fluorescence microscopy of GFP in different organs or tissues. For tail vein injection, 200 µg of each recombinant protein were used. After injection for 4 h, mice were sacrificed by deep anesthesia, perfused with 100 ml PBS and then fixed with 4% PFA. Using a cryostat, tissues or organs were cut into 8–10-µm thick slices. Slices mounted on the slide were stained with DAPI. (B) The heat map presenting quantitative analysis of GFP fluorescence intensity in GFP and GFP-Dot1l group. (C) The heat map presenting quantitative analysis of GFP fluorescence intensity in 36 + GFP and 36 + GFP-Dot1l group.