Skip to main content
. 2022 Jan 24;11(1):368–383. doi: 10.1080/22221751.2022.2026741

Figure 6.

Figure 6.

Immune responses after COVID-19mRNA vaccination and virus challenge in aged and young mice. (A) Vaccination-induced serum neutralizing antibody titre against SARS-CoV-2 B.1.1.7 at day 14, 28 after the first dose of vaccine. (B) Serum-neutralizing antibody titre against SARS-CoV-2 B.1.1.7 at day 2 post-virus challenge. Data represent mean ± SEM. n = 6 for each group. **P < 0.01 by Student’s t-test. (C) Viral-specific IgG-producing cells were detected by in vitro stimulation of spleen single cells suspension with inactivated SARS-CoV-2 virus for 48 h. IgG-producing cells were visualized by staining with a mouse IgG ELISPOT kit. (D) Interferon-γ-producing cell responses in the spleens collected at 2 dpi from vaccinated mice. Viral-specific interferon-γ producing cells were detected by in vitro stimulation of single-cell suspension sample with SARS-CoV-2 RBD peptide pool and NP protein for 48 h and then visualized by staining with a mouse IFN-γ ELISPOT kit. Data represent mean ± SD. n = 3 for each group. **P < 0.01 by Student’s t-test.