Skip to main content
. 2022 Jan 24;11(1):368–383. doi: 10.1080/22221751.2022.2026741

Table 1.

Sequences of primers and probes for real time RT-qPCR detection of viral load and mRNA gene expression of host cytokines/chemokines.

Gene name Forward primer (5’ to 3’) Reverse Primer (5’ to 3’)
SARS-CoV-2 RdRp CGCATACAGTCTTRCAGGCT GTGTGATGTTGAWATGACATGGTC
Probe (5’ to 3’): FAM- TTAAGATGTGGTGCTTGCATACGTAGAC-lABkFQ
β-actin ATGGCCAGGTCATCACCATTG CAGGAAGGAAGGCTGGAAAAG
Probe (5’ to 3’): Cy5-AGCGGTTCCGTTGCCCTGAG-IABkFQ
IL-1β GCCTTGGGCCTCAAAGGAAAGAATC GGAAGACACAGATTCCATGGTGAAG
IL-6 TGGAGTCACAGAAGGAGTGGCTAAG TCTGACCACAGTGAGGAATGTCCAC
TNF-α ATAGCTCCCAGAAAAGCAAGC CACCCCGAAGTTCAGTAGACA
IFN-α ARSYTGTSTGATGCARCAGGT GGWACACAGTGATCCTGTGG
IFN-γ AAGCGTCATTGAATCACACC CGAATCAGCAGCGACTCCTT
IP-10 ATGACGGGCCAGTGAGAATG GAGGCTCTCTGCTGTCCATC