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(A) Procedure for isolation, culture and encapsulation of HAP stem cells. (B) Procedure for implantation of PFM-encapsulated HAP stem cells. Experiment I: GFP-expressing PFM-encapsulated HAP stem cells from C57BL/6J GFP mice were implanted in the severed thoracic spinal cord of nude mice with SCI three week after injury. Experiment II: PFM-encapsulated HAP stem cells of C57BL/6J mice without GFP expression were implanted in C57BL/6J mice with SCI.
