FIG. 1.

Primer design strategy for the forward and reverse PCR primers (primers MP-F and MP-R, respectively) indicating the binding positions within the M. pneumoniae genome (10). The primer sequences are shown at the bottom of each panel. Shaded areas indicate mismatches between the primer and the genome sequences within REPMP2/3 regions. The P1 adhesin gene sequence (P1) is indicated by the appropriate genome sequence. (A) Alignment of forward primer MP-F; (B) alignment of reverse primer MP-R.