FIGURE 1 |.
Mutual impacts on subclonal growth. (A) 168FARN and 4T07 parental cells were transduced either with an empty retroviral vector (168P and 4T07P) or with labeled with a GFP-encoding retrovirus (168G and 4T07G). Cells were seeded in triplicate in six-well plates at a density of 50,000 cells/well and cultured for the indicated times before harvesting and counting. (B) 105 Cells were seeded at a 1:1 ratio in homotypic (parental and GFP expressing derivative of the same cell line) or heterotypic (different cell lines, one expressing GFP) co-cultures and harvested and replated at the initial densities (105 cells/plate) at indicated times. The ratios of GFP-labeled to unlabeled cells were estimated by flow cytometry. The results represent data from three independent experiments and are shown as mean ± SEM.