FIGURE 6 |.

Extracellular β-hydroxybutyrate leads to increased H3K9 histone acetylation and altered gene expression in 4T07 cells. (A) Expression levels of the slc16A family transporter genes in 4T07 were analyzed by RT-QPCR. Expression of HPRT served as normalization of the data. (B) H3K9 histone acetylation was analyzed by immunoblotting of extracts of 4T07 cells grown for 24 h in control, 168-conditioned medium or medium complemented with β-hydroxybutyrate or with butyrate, as indicated. Total histone 3 (H3) abundance served as normalization control. (C) 4T07 cells cultured for 3 days were incubated for 8 h with 4T07- (Ctrl) or 168-conditioned medium or purified β-hydroxybutyrate (10 mM) added to fresh medium. Total RNAs were purified and subjected to RT-QPCR with specific primers for LCN2 and IL-11. **p < 0.01, ***p < 0.001.