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. 2022 Jan 10;11:e69906. doi: 10.7554/eLife.69906

Key resources table.

Reagent type (species) or resource Designation Source or reference Identifiers Additional information
Antibody anti-METTL3(Rabbit monoclonal) Cell Signaling Technology Cat# 96,391 WB (1:1000)
Antibody anti-METTL14(Rabbit monoclonal) Cell Signaling Technology Cat# 51,104 WB (1:1000)
Antibody anti-METTL16(Rabbit monoclonal) Cell Signaling Technology Cat# 17,676 WB (1:1000)
Antibody anti-WTAP(Rabbit monoclonal) Cell Signaling Technology Cat# 56,501 WB (1:1000)
Antibody anti-Virillizer(Rabbit monoclonal) Cell Signaling Technology Cat# 88,358 WB (1:1000)
Antibody anti-phospho-EGFR(Rabbit monoclonal) Cell Signaling Technology Cat# 3,777 WB (1:1000)
Antibody anti-EGFR(Rabbit monoclonal) Cell Signaling Technology Cat# 4,267 WB (1:1000)
Antibody anti-VCAM-1(Rabbit monoclonal) Cell Signaling Technology Cat# 15,631 WB (1:1000)
Antibody anti-VCAM-1(Rabbit monoclonal) Cell Signaling Technology Cat# 39,036 IF (1:100)
Antibody anti-thrombospondin-1(Rabbit monoclonal) Cell Signaling Technology Cat# 37,879 WB (1:1000)
Antibody anti-αSMA(Rabbit monoclonal) Cell Signaling Technology Cat# 19,245 WB (1:1000)
Antibody anti-phospho-ERK(Rabbit monoclonal) Cell Signaling Technology Cat# 8,544 WB (1:1000)
Antibody anti-phospho-AKT(Rabbit monoclonal) Cell Signaling Technology Cat# 5,012 WB (1:1000)
Antibody anti-ERK(Mouse monoclonal) Santa Cruz Cat# sc-271269 WB (1:1000)
Antibody anti-AKT(Mouse monoclonal) Santa Cruz Cat# sc-5298 WB (1:1000)
Antibody anti- METTL3(Rabbit monoclonal) Proteintech Cat# 15073–1-AP IF (1:100)
Antibody anti- GFP(Rabbit monoclonal) Proteintech Cat# 50430–2-AP IF (1:100)
Antibody anti- GAPDH (Rabbit monoclonal) Proteintech Cat# 60004–1-Ig WB (1:5000)
Antibody anti- EGFR (Rabbit monoclonal) Abcam Cat# ab52894 IF (1:100)
Antibody anti- VE-cadherin (Rat monoclonal) Abcam Cat# ab33168 IF (1:100)
Antibody anti- CD31(Rabbit monoclonal) Abcam Cat# ab24590 IF (1:100)
Antibody anti- CD68(Rabbit monoclonal) Abcam Cat# ab955 IF (1:100)
Antibody anti- vWF(Sheep monoclonal) Abcam Cat# ab11713 IF (1:100)
Antibody anti-thrombospondin-1(Mouse monoclonal) Abcam Cat# ab1823 IF (1:100)
Antibody anti- thrombospondin-1 (Mouse monoclonal) Abcam Cat# ab1823 IF (1:100)
Antibody Alex 488-conjugated goat anti-rabbit antibody Thermo Fisher Scientific Cat# A-11008 IF (1:200)
Antibody Alex 594-conjugated goat anti-mouse antibody Thermo Fisher Scientific Cat# A-11008 IF (1:200)
Antibody Alex 488-conjugated goat anti-rabbit antibody Thermo Fisher Scientific Cat# A-11005 IF (1:200)
Antibody Alex 594-conjugated donkey anti-sheep antibody Thermo Fisher Scientific Cat# A-11016 IF (1:200)
Chemical compound, drug AG1478 Selleck Cat# S2728
Chemical compound, drug Recombinant Human Thrombospondin-1 Absin Cat# abs 04665 1.03 mg/ml
sequence-based reagent Human EGFR-3utr This paper N/A Sequences in Supplementary file 3
sequence-based reagent Human EGFR This paper N/A Sequences in Supplementary file 3
sequence-based reagent Human THBS1 This paper N/A Sequences in Supplementary file 3
sequence-based reagent Human GAPDH This paper N/A Sequences in Supplementary file 3
software, algorithm Ingenuity Pathway Analysis National Clinical Research Center for Blood Diseases http://www.ingenuity.com/
software, algorithm Prism version 8.0 GraphPadSoftware Inc https://www.graphpad.com/scientific-software/prism/