Figure 1.

Aloin inhibited HCC cell proliferation and invasion and induced apoptosis and autophagy. Human HCC cell lines HuH7 and HCCLM3 were treated with different concentration of aloin (0, 10, 30, 50, 70 μmol/L). (a, b) Cell viability was determined by CCK-8 assay at different time points (24, 48, 72 h). (c, d) Cell apoptosis ratios were measured by flow cytometry. (e) Effects of aloin on HCC cell invasion ability were analyzed using transwell invasion assay. (f, g) Relative expressions of apoptosis-related protein (BCL2, BAX, MMP2) were detected by Western blot. (h, i) Relative expressions of autophagy-related protein (P62, Beclin-1, LC3 I, LC3 II) were detected by Western blot. Data were presented as mean±SEM (n ≥ 3). *P < 0.05, **P < 0.01, ***P < 0.001 versus 0 μmol/L or control