Figure 3.

Circ_0011385 served as a sponge for miR-149-5p. (a) Predicted binding regions of miR-149-5p with WT or MUT circ_0011385. Cells were transfected with miR-149-5p mimics or miR-NC (negative control) (b) Transfection efficiency of miR-149-5p mimics plasmid was measured by qRT-PCR in HuH7 and HCCLM3 cells. (c) The interaction of circ_0011385 and miR-149-5p was verified by dual-luciferase reporter assay in HEK-293 T cells (*P < 0.05 vs. miR-NC group). (d) Ago2 RNA immunoprecipitation (RIP) assay was used for detection of the amount of miR-149-5p and circ_0011385 in HEK-293 T cells (*P < 0.05 vs. IgG group). (e) HuH7 and HCCLM3 were interfered as indicated with circ_0011385 siRNA or miR149-5p inhibitors. Relative expression of miR-149-5p was shown by qPCR detection. Data were presented as mean±SEM (n ≥ 3)