Figure 6.

MiR-149-5p suppressed the development of HCC via WT1. Cells were introduced with WT1 expression plasmid (pcDNA was used as negative control). (a) Relative expressions of WT1 in HCCLM3 and HuH7 determined by Western Blot assay (p*<0.05 versus pcDNA). Cells were successively treated with or without WT1 expression plasmid or pcDNA and miR-149-5p mimics. (b, c) Cell viability was determined by CCK-8 assay. (d) Cell apoptosis ratios were measured by flow cytometry. (e) Effects on cell invasion ability were determined using transwell invasion assay. (f, g) Relative expressions of apoptosis-related protein (BCL2, BAX, MMP2) was detected by Western blot, GADPH was used as the internal reference. (h, i) Relative expressions of autophagy-related protein (P62, Beclin-1, LC3 I, LC3 II) detected by Western blot, GADPH was used as internal reference. Data were presented as mean±SEM (n ≥ 3). *P < 0.05 versus “miR-NC” or “miR-149-5p+pcDNA”