Figure 8.

Aloin exerted tumor inhibitory effect via regulating circ_0011385/miR149-5p/WT1 axis in vivo. The HuH7 cells with or without shRNA treatment were subcutaneously injected into the left fank of BALB/c nude mice and allowed to grow for 5 weeks, saline or aloin (40 mg/kg) were given by intraperitoneal injection every day. (a) The tumor volume was measured every 7 days. (b) Tumors were removed and weighed on the 35th day. (d) qRT-PCR analysis of miR-149-5p expression in tumor tissue. (e) Immuno-blotting analysis of WT1 expression in tumor tissue. (f) Immunohistochemistry assay of BCL2. (g) Relative expressions of apoptosis-related protein (BCL2, BAX, MMP2) and autophagy-related protein (P62, Beclin-1, LC3 I, LC3 II) were detected by Western blot, GADPH was used as internal reference. Data were presented as mean±SEM (n ≥ 3). *P < 0.05 versus control and aloin +sh-NC