Figure 1.

MiR-132-3p was lowly expressed, and reduced cell apoptosis in SH-SY5Y cells after the treatment of Aβ1-42

(a) The viability of SH-SY5Y cells after the treatment of Aβ1-42 at different doses (0, 2.5, 5 and 10 μM) was tested by MTT assay. One way ANOVA followed by Dunnett’s post hoc test. (b) The expression of miR-132-3p in SH-SY5Y cells treated by different doses of Aβ1-42 (0, 2.5, 5 and 10 μM). One way ANOVA followed by Dunnett’s post hoc test. (c) The expression of miR-132-3p in SH-SY5Y cells after treatment of miR-132-3p mimics and Aβ1-42. One way ANOVA followed by Tukey’s post hoc test. (d) The MTT assay showed the viability of SH-SY5Y cells in control, Aβ1-42 (10 μM), Aβ1-42+ NC mimics and Aβ1-42+ miR-132-3p mimics groups. One way ANOVA followed by Tukey’s post hoc test. (e) TUNEL assay was used to measure cell SH-SY5Y cell apoptosis in the four groups. One way ANOVA followed by Tukey’s post hoc test. (f-g) The protein levels of Bax, Bcl-2, cleaved-caspase-3, APP and BACE1 were assessed in the indicated groups using western blot. One way ANOVA followed by Tukey’s post hoc test. N = 3 for each assay. *p < 0.05, **p < 0.01, ***p < 0.001.