Figure 2.

MiR-132-3p negatively regulated HNRNPU in SH-SY5Y cells

(a) The mRNAs for miR-132-3p were predicted on the starBase website under conditions of CLIP-Data ≥ 5, pan-Cancer ≥ 2, program Num ≥ 1, program: microT, miRanda, miRmap, PITA, PicTar, TargetScan. (b) The expression of mRNAs was examined after overexpressing miR-132-3p. Student’s t test. (c) The HNRNPU mRNA and protein expression in Aβ1-42-stimulated SH-SY5Y cells. One way ANOVA followed by Dunnett’s post hoc test. (d) The starBase website predicted the binding sequences between HNRNPU and miR-132-3p. (e) A luciferase reporter assay was used to explore the relationship of HNRNPU and miR-132-3p. Student’s t test. (f) The HNRNPU mRNA and protein levels in SH-SY5Y cells posttransfection of miR-132-3p mimics was detected using RT-qPCR and western blot analyses. Student’s t test. N = 3 for each assay. *p < 0.05, **p < 0.01, ***p < 0.001.