Figure 3.

HNRNPU regulated BACE1 expression by acting as an RBP

(a) HNRNPU, as an RBP, can bind with BACE1 mRNA, which was predicted on the starBase website. (b) The BACE1 mRNA and protein levels in SH-SY5Y cells stimulated by increased concentrations of Aβ1-42 were measured using RT-qPCR and western blot analyses. One way ANOVA followed by Dunnett’s post hoc test. (c) The binding ability between HNRNPU and BACE1 in SH-SY5Y cells was identified by RNA pull down assay using Bio-BACE1 sense. (d) RIP assay further confirmed the enrichment of BACE1 pulled down by anti-HNRNPU in SH-SY5Y cells. Student’s t test. (e) The downregulation of HNRNPU in SH-SY5Y cells post transfection of sh-HNRNPU. Student’s t test. (f) The expression of BACE1 mRNA in SH-SY5Y cells treated with actinomycin D after indicated transfection. Two way ANOVA. (g) The BACE1 mRNA levels in SH-SY5Y cells after indicated transfection. Student’s t test. (h) The BACE1 protein levels in SH-SY5Y cells after indicated transfection. One way ANOVA followed by Tukey’s post hoc test. N = 3 for each assay. *p < 0.05, **p < 0.01, ***p < 0.001.