Figure 5.

LIFR-AS1 serves as a ceRNA that sequesters miR-150-5p and thus alters the expression of VEGFA in PC cells

(a, b) VEGFA expression was quantified via qRT-PCR and Western blotting in PANC1 and Mia-capa2 cells transfected using si-LIFR-AS1 or control constructs. (c) Following transfection with antagomir-150-5p or antagomir-NC, miR-150-5p levels in PC cell lines were confirmed via qRT-PCR. (d, e) PC cell lines were co-transfected with si-LIFR-AS and antagomir-150-5p or antagomir-NC after which VEGFA levels were measured via qRT-PCR and Western blotting. *P < 0.05; **P < 0.01; ***P < 0.001; #P > 0.05.