Figure 6.
Senescence and apoptosis of PFFs of different sizes. A. SA-β-gal-stained PFFs of different sizes. B. The apoptotic rates of different sized PFFs as assessed by flow cytometry. Magnification, 10×. PI and Annexin V staining was used to determine the percentage of live (PI−/Annexin V-FITC−), early apoptotic (PI−/Annexin V-FITC+) and apoptotic or necrotic (PI+/Annexin V-FITC+) cells. C. Quantitative data of senescence in different sized PFFs. D. Quantitative data of apoptosis in PFFs of different sizes. Data are presented as the mean±SD of three independent experiments (a, b, cP<0.05)