Figure 1.

Alix, TSG101 and HSC70 protein levels were upregulated in exosomes derived from PCa cells and PCa patient serum. (a) Electron microscopic images of exosomes isolated from PCa cells. (b-e) The protein levels of Alix, TSG101 and HSC70 in the exosomes of normal prostatic epithelial cells (BPH1 and RWPE1) and PCa cells (VCap and LNCap, DU145, PC3) were determined by Western blot assay. *P < 0.05 compared with BPH1-Exo; ^#P < 0.05 compared with RWPE1-Exo group. (f and g) The protein levels of Alix, TSG101 and HSC70 were measured in the exosomes derived from serum of healthy volunteers (n = 10) and PCa patients (n = 10) with Western blot assay. *P < 0.05 compared with normal-Exo group. N = 10, data were expressed as mean ± SEM; Student’s t test or one-way ANOVA was used for analyzing data