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. 2021 Nov 1;20(23):2531–2546. doi: 10.1080/15384101.2021.1992853

Figure 4.

Figure 4.

Effect of overexpression or knockdown of lncAY927529 on the level of lncAY927529 in PCa cells and exosomes derived from PCa cells. LncAY927529 siRNA, scramble (a negative control of lncAY927529 siRNA), pcDNA-lncAY927529 and pcDNA3.1 (a negative control of pcDNA-lncAY927529) were transfected into PC3 and DU145 cells: (a and b) LncAY927529 level was detected in PC3 and DU145 cells with RT-qPCR transfected with lncAY927529 siRNA or scramble. *P < 0.05 compared with scramble group. (c and d) LncAY927529 level was detected in PC3-Exo and DU145-Exo with RT-qPCR when PC3 and DU145 cells were transfected with lncAY927529 siRNA or scramble. *P < 0.05 compared with scramble-Exo group. (e and f) LncAY927529 level was detected in PC3 and DU145 cells with RT-qPCR transfected with pcDNA-lncAY927529 or pcDNA3.1. *P < 0.05 compared with pcDNA3.1 group. (g and h) LncAY927529 level was detected in PC3-Exo or DU145-Exo with RT-qPCR when PC3 and DU145 cells were transfected with pcDNA-lncAY927529 or pcDNA3.1. *P < 0.05 compared with pcDNA3.1-Exo group. N = 4, data were expressed as mean ± SEM; Student’s t test or one-way ANOVA was used for analyzing data