Figure 8.

Prediction and verification the relationship between CXCL14 and lncAY927529. RPIseq (http://ctsb.is.wfubmc.edu/projects/rpi-pred/rpipred1.php) (a) and Pull down assay (b) were used to predict and verify the relation of lncAY927529 and CXCL14. *P < 0.05 compared with control or scramble AY927529 group. (c and d) Western blot assay was used to detected CXCL14 and ERK phosphorylated protein levels in ST2 cells treated with exosomes derived from DU145 cells with knockdown of AY927529 or overexpression of AY927529 for 48 h, and the conditioned medium (ST2-CM) was collected. *P < 0.05 compared with DU145-pcDNA3.1-Exo group or DU145-scramble-Exo group. N = 4, data were expressed as mean ± SEM; Student’s t test or one-way ANOVA was used for analyzing data