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. 2022 Jan 20;2022:6603296. doi: 10.1155/2022/6603296

Figure 5.

Figure 5

CD147 augmented pressure overload-induced oxidative stress and ferroptosis in a glycosylation-dependent manner. (a) Representative immunostaining for 4-hydroxynonenal (4-HNE) and nitrotyrosine (NT) in mouse myocardia from the indicated groups. (b) Representative immunoblotting and (c) statistical analysis for NT, 4-HNE, and NOX4 protein expression. Results were normalised to GAPDH levels (n = 5–6 mice/group). (d) Representative western blotting of ferroptosis-related proteins, including ACSL4, NOX1, COX-2, and GPX4; GAPDH served as the loading control (n = 5–6 mice/group). (e) Quantitative analysis of ferroptosis-related gene expression by real-time polymerase chain reaction or protein expression by western blot in the different groups. ∗P < 0.05 vs. the sham group; ∗∗P < 0.01 vs. the sham group; ∗∗∗P < 0.001 vs. the sham group; ∗∗∗∗P < 0.0001 vs. the sham group; #P < 0.05 vs. the vehicle group; ##P < 0.01 vs. the vehicle group; ✟P < 0.05 vs. the OE group; ✟✟P < 0.01 vs. the OE group. 4-HNE: 4-hydroxynonenal; NT: nitrotyrosine; NOX4: NADPH oxidase 4; ACSL4: acyl-CoA synthetase long-chain family member 4; NOX1: NADPH oxidase 1; COX-2: cyclooxygenase-2; GPX4: glutathione peroxidase 4.