Figure 1.

PLP inhibited PRV infection within PK15 cells. We pretreated PK15 cells using PLP at diverse doses for a 1 h period prior to PRV XJ5 (MOI = 0.1) infection. We later cultured cells with 2% DMEM that contained PLP for a 24 h period. (a) At 12 and 24 hpi, the changes in cell morphology were observed under a microscope (b). At 24 hpi, β-actin and PRV gB protein levels were measured by WB assay. (c) Viral titers were determined by TCID_50. (d) Internalized virus was evaluated by IFA. The error bars indicated the SD of 3 separate assays. ^∗∗P < 0.01.