Figure 3.

PLP inhibits PRV attachment and penetration into PK15 cells. (a) We treated PK15 cells by PLP at diverse doses for a 1 h period and then infected by PRV XJ5 (MOI = 0.1) with PLP. At 24 hpi, we conducted WB assay for evaluating β-actin and PRV gB protein levels. (b) We infected PK15 cells by PRV XJ5 (MOI = 0.1) for a 1 h period under 4°C with PLP at diverse doses and incubated with different concentrations of PLP at 37°C for a 1 h period prior to removal of PLP DMEM as well as cultivated within PLP-free DMEM. Protein levels of β-actin and PRV gB were measured through WB at 24 hpi. (c) We determined virus titers through TCID₅₀. (d) We assessed internalized virus through IFA. The error bars indicated SD of 3 individual assays. ^∗P < 0.05, ^∗∗P < 0.01.