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. 2022 Jan 20;2022:2522249. doi: 10.1155/2022/2522249

Figure 5.

Figure 5

miR-338-3p targeted CARM1. (a) The binding sites between CARM1 3'UTR and miR-338-3p were shown. The interaction between CARM1 and miR-338-3p was verified by dual-luciferase reporter assay (b) and RIP assay (c). (d, e) The mRNA and protein expression of CARM1 in the serum of DR patients and normal control volunteers was detected by qRT-PCR and WB analysis. (f) The correlation between CARM1 and miR-338-3p was analyzed by Pearson's correlation coefficient analysis. (g) The CARM1 protein expression in ARPE-19 cells under HG and NG conditions was determined by the WB analysis. (h) The miR-338-3p expression was detected by qRT-PCR to evaluate the transfection efficiency of miR-338-3p mimic and inhibitor. (i) The protein expression of CARM1 was detected by the WB analysis in HG-induced ARPE-19 cells transfected with miR-338-3p mimic and inhibitor. ∗∗P < 0.01, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001.