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. 2022 Jan 20;2022:2522249. doi: 10.1155/2022/2522249

Figure 7.

Figure 7

Effects of CARM1 and miR-338-3p on HG-induced ARPE-19 cell injury. (a) The transfection efficiency of pcDNA CARM1 overexpression vector was assessed by detecting CARM1 protein expression using the WB analysis. (b–j) ARPE-19 cells were transfected with miR-NC, miR-338-3p, miR-338-3p + pcDNA, or miR-338-3p + CARM1 and then treated with HG. The cells treated with NG were used as control. (b) The WB analysis was used to measure the protein expression of CARM1. (c) The concentrations of IL-6 and TNF-α were examined using ELISA. Cell proliferation and apoptosis were measured by MTT assay (d), EdU assay (e), and flow cytometry (f, g). (h) The WB analysis was used to detect the protein expression of Bax and cleaved-caspase-3. (i, j) MDA level and SOD activity were determined to assess cell oxidative stress. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001.