Fig. 1. Experimental design.

Six-week-old C57BL/6 mice were placed on a HFD for 8 weeks then subjected to brown adipose tissue transplantation (BAT-Tx). Sixteen-weeks post BAT-Tx transplantation mice were subjected to either a Sham or myocardial infarction (MI) surgery. Two weeks before MI, mice were subjected to echocardiography and body composition assessment. At 4, 10, and 22 post MI, mice were subjected to body composition assessment, and glucose tolerance tests (GTT). Insulin tolerance tests (ITT) and exercise tolerance tests were performed at 21 weeks post MI, and echocardiography was performed at 22 weeks post MI. At 22 weeks post MI, mice were euthanized, and tissues were collected for assessment of gene expression and biochemical methods.