Table 1.
Binding affinity of ELP-GFP constructs for serum albumin
| Construct | ISAz treated | KD MSA (μM) | KD HSA (μM) |
| ELP(0FA)-GFP | No | n.d. | n.d. |
| ELP(1FA)-GFP | No | 126 ± 32.2 | n.a. |
| ELP(5FA)-GFP | No | 10.3 ± 4.0 | n.a. |
| ELP(10FA)-GFP | No | 2.76 ± 0.19 | n.a. |
| ELP(1FA)-GFP | Yes | 25.9 ± 7.1 | 19.3 ± 3.9 |
| ELP(5FA)-GFP | Yes | 4.0 ± 1.6 | 3.16 ± 0.60 |
| ELP(10FA)-GFP | Yes | 2.22 ± 0.03 | 1.64 ± 0.17 |
The KD values were calculated from the steady-state affinity binding model obtained by SPR (Materials and Methods). Mean ± SD values were derived from four to eight independent experiments. n.d. indicates that no binding was detected and n.a. indicates not analyzed.