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AMP-PNP supports preloading of CMG onto forked DNA. (A) Scheme of the assay. Preincubation for CMG loading was with 0.3 mM ATPγS or 0.3 mM AMP-PNP after which 5 mM ATP was added. (B) PAGE analysis of products during the preincubation with either ATPγS or AMP-PNP, and after the addition of ATP. (C) Quantitation of triplicate assays using ATPγS in which the error bars on the data points represent the SEM.
