Fig. 5.

CMG is less able to unwind long products with ATPγS compared with the use of ATP. (A) Unwinding reactions were performed using either ATP or ATPγS. (B) Substrate and products using ATPγS were analyzed by native PAGE (horizontal slices are shown), and then scanned for intensity by a Typhoon phosphorimager (arbitrary units, but the same units are used for both gels in B and C). (C) The same as B except for use of ATP instead of ATPγS. (D) Quantitation shows that as the length of the duplex increases, the ratio of DNA that is unwound using ATPγS compared with the unwound DNA using ATP becomes smaller (i.e., ATP results in greater unwinding than ATPγS). The red dashed lines through B and C point out the unwound DNA peaks. The ratio of the areas of these peaks is presented in D.