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. 2022 Jan 24;119(4):e2100679119. doi: 10.1073/pnas.2100679119

Fig. 1.

Fig. 1.

Soluble EGF disrupts E-cadherin/EGFR complexes. (A and B) Co-IP results of EGFR and E-cadherin obtained with A-431DE-cad monolayers. Cells were seeded on PDMS membranes coated with fibronectin and cultured in reduced serum (0.5 vol%) for 24 h prior to treatment with 20 nM EGF for 15 min. Controls were not treated with EGF. Lysates were pulled down with (A) anti-EGFR antibody or (B) anti–E-cadherin and probed for respectively, E-cadherin or EGFR. (C) Quantified, normalized band intensities obtained under conditions in panel A (n = 4) and panel B (n = 3). Results were normalized by the band intensities in untreated samples. (D and E) Co-IP measurements of MCF-10A monolayers. Immunoprecipitation was done with either (D) anti-EGFR or (E) anti–E-cadherin antibody. (F) Band intensities, normalized as in panel C, were determined for anti-EGFR (n = 3) and anti-E-cadherin (n = 4) pulldowns. Error bars are SEM. *P < 0.05, **P < 0.005, ***P < 0.0005.