Fig. 3.
EGF and cyclic stretch cooperate to disrupt E-cadherin/EGFR complexes. Co-IP results obtained with 24 h serum-starved confluent A-431DE-cad monolayers subjected to four conditions: ±3 nM EGF and ±10% cyclic stretch for 30 min. EGF-neutralizing antibody was added to all samples not treated with EGF. (A) Co-IP results for monolayers seeded on E-cad-Fc–coated PDMS membranes for 5 h in the presence of 16G3 antibody. (B) Normalized E-cadherin band intensities obtained from Co-IP measurements in panel A (n = 3). (C) Co-IP results obtained with confluent A-431DE-cad cells on fibronectin-coated PDMS. (D) Normalized E-cadherin band intensities for measurements shown in C (n = 3). Error bars are SEM. *P < 0.05, **P < 0.005, ***P < 0.0005.